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The sharpness of the boundaries which have been shaped serves being an index with the heterogeneity in the sample. The first analytical ultracentrifuges have been designed and built by T. Svedberg during the twenties.
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The mixture is then sent to the centrifuge tube. Applying this method, a density gradient is shaped that decreases linearly for a purpose of quantity in between the limiting densities initially existing in The 2 cylinders.
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The counterpart to analytical centrifugation is preparative centrifugation, which provides to the isolation of cell parts for additional Assessment.
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Differential centrifugation has many major disadvantages. Since the homogenate is to begin with distributed uniformly through the entire centrifuge tube, the 1st particles sedimented will always be contaminated with smaller quantities of all other constituents in the homogenate. This influence is revealed in Figure 12-5.
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